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Influenza specific activities
Influenza activities are articulated around different themes:
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Laboratory assessment
- Assessment of Influenza laboratories, from regional to National Influenza Center
- Development of a computerized specific assessment tool for Influenza laboratories, currently under development.
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Laboratory facility design
- Design or redesign of Influenza laboratory
- Feasibility surveys, cost estimates
- BSL2+/BSL3 design
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Technical assistance and expertise specific to Influenza and respiratory diseases
- Laboratory premises recommendations
- Development and adaptation of diagnosis methodology
- Help in adapting or developing SOP and general procedures
- Technical training on molecular biology diagnosis of influenza and others respiratory viruses (PCR, RT PCR, multiplex)
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Specific skills, certificate, training and experience of the IQLS staff:
- Mr Orelle, former deputy director of the National Influenza Center for Madagascar (2011-2013)
- 9th Virology – HKU-Pasteur Research Center, Hong-Kong, SAR China-2012
- 4th Immunology – HKU- Pasteur Research Center, Hong-Kong, SAR China, 2011
- Bioinformatics and sequencing on influenza – CDC workshop (NICD), Johannesburg-South Africa, 2011
- Influenza antiviral susceptibility surveillance WHO training course, Health Protection Agency (HPA), London, United Kingdom, 2010
- Viral culture and titration (hemagglutination, inhibition of hemagglutination)
- Molecular diagnosis (pandemic and seasonal influenza)
- Human and swine respiratory organ ex vivo culture models
- Molecular biology PCR, RT-PCR, qPCR, qRT-PCR, RNA and DNA extraction (manual and automated), multiplex
- Development of real-time PCR techniques for virological diagnosis